Considerações sobre o uso do piercing lingual

Objetivo: Verificar as implicações do uso do piercing lingual em indivíduos da cidade de Araraquara, São Paulo. Métodos: A cidade de Araraquara, São Paulo possui cinco estabelecimentos para colocação de piercing, dos quais apenas dois autorizaram a realização da pesquisa e permitiram acesso a listagem dos usuários de piercing lingual. Os usuários foram contatados pelo telefone. Ao final do estudo a amostra esteve composta por 100 indivíduos de ambos os sexos, maiores de 18 anos, que fazem ou fizeram uso do piercing lingual. Para levantamento das informações utilizou-se um formulário, com seis questões objetivas. Os dados obtidos foram analisados por meio de estatística descritiva. Resultados: Dos participantes, 77 indivíduos usavam piercing lingual há mais de seis meses. O tempo de cicatrização após a colocação do adereço foi abaixo de quatro meses, sendo que, 68 pessoas utilizaram enxaguatórios bucais e 19, além deste, utilizaram analgésicos. Apenas 11 pessoas não apresentaram sintomas após as primeiras semanas decorrentes da colocação do piercing e 56 indivíduos relataram complicações como aumento da salivação, dificuldade na fala, fraturas dentárias, traumas na gengiva ou mucosa e dificuldades na alimentação. Conclusão: Frente às implicações apresentadas pelo uso do piercing lingual justifica-se o despendimento de esforços na orientação de jovens frente à opção de utilização de piercing na cavidade oral.Objective: Investigated the consequences of tongue piercing in individuals from the city of Araraquara, São Paulo, Brazil. Methods: In Araraquara there are five piercing shops, but only two agreed to help the research by providing the contact information of customers who had had their tongues pierced. These customers were contacted by telephone. By the end of the research, the sample consisted of 100 individuals from both genders aged 18 years or more. Not all of them still had their tongue piercing. A form with six objective questions was used to collect information. The information was then analyzed by descriptive statistics. Results: From the 100 participants, 77 had been using tongue piercing for more than 6 months. It took less than 4 months for the tongue to heal. Sixty–eight individuals used oral rinses and of these, 19 also used painkillers. Only 11 individuals did not present symptoms after the first weeks using the piercing. On the other hand, 56 individuals reported complications such as increased salivation, speaking difficulties, gingival or mucosal traumas, dental fractures and eating difficulties. Conclusion: All these tongue-piercing complications justify efforts to inform the youth about the use of piercing in the oral cavity

Bacterial cellulose-collagen nanocomposite for bone tissue engineering

A nanocomposite based on bacterial cellulose (BC) and type I collagen (COL) was evaluated for in vitro bone regeneration. BC membranes were modified by glycine esterification followed by cross-linking of type I collagen employing 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Collagen incorporation was studied by spectroscopy analysis. X-Ray diffraction showed changes in the BC crystallinity after collagen incorporation. The elastic modulus and tensile strength for BC-COL decreased, while the strain at failure showed a slight increase, even after sterilization, as compared to pristine BC. Swelling tests and contact angle measurements were also performed. Cell culture experiments performed with osteogenic cells were obtained by enzymatic digestion of newborn rat calvarium revealed similar features of cell morphology for cultures grown on both membranes. Cell viability/proliferation was not different between BC and BC-COL membranes at day 10 and 14. The high total protein content and ALP activity at day 17 in cells cultured on BC-COL indicate that this composite allowed the development of the osteoblastic phenotype in vitro. Thus, BC-COL should be considered as alternative biomaterial for bone tissue engineering.FAPESP [08-58776-6, 09/09960-1]FAPESPCNPqCNP

Biopolymer-based membranes associated with osteogenic growth peptide for guided bone regeneration

Barrier membranes for guided bone regeneration (GBR) mainly promote mechanical maintenance of bone defect space and induce osteopromotion. Additionally, biopolymer-based membranes may provide greater bioactivity and biocompatibility due to their similarity to extracellular matrix (ECM).In this study, biopolymers-based membranes from bacterial cellulose (BC) and collagen (COL) associated with osteogenic growth peptide (OGP(10–14)) were evaluated to determine in vitro osteoinductive potential in early osteogenesis; moreover, histological study was performed to evaluate the BC–COL OGP(10–14) membranes on bone healing after GBR in noncritical defects in rat femur. The results showed that the BC–COL and BC–COL OGP(10–14) membranes promoted cell proliferation and alkaline phosphatase activity in osteoblastic cell cultures. However, ECMmineralization was similar between cultures grown on BC OGP(10–14) and BC–COL OGP(10–14) membranes. In vivo results showed that all the membranes tested, including the peptide-free BC membrane, promoted better bone regeneration than control group. Furthermore, the BC–COL OGP(10–14) membranes induced higher radiographic density in the repaired bone than the other groups at 1, 4 and 16 weeks. Histomorpho-metric analyses revealed that the BC–COL OGP(10–14) induced higher percentage of bone tissue in the repaired area at 2 and 4 weeks than others membranes. In general, these biopolymer-based membranes might be potential candidates for bone regeneration applications

Strong Electronic Identification: Survey & Scenario Planning

The deployment of more high-risk services such as online banking and government services on the Internet has meant that the need and demand for strong electronic identity is bigger today more than ever. Different stakeholders have different reasons for moving their services to the Internet, including cost savings, being closer to the customer or citizen, increasing volume and value of services among others. This means that traditional online identification schemes based on self-asserted identities are no longer sufficient to cope with the required level of assurance demanded by these services. Therefore, strong electronic identification methods that utilize identifiers rooted in real world identities must be provided to be used by customers and citizens alike on the Internet. This thesis focuses on studying state-of-the-art methods for providing reliable and mass market strong electronic identity in the world today. It looks at concrete real-world examples that enable real world identities to be transferred and used in the virtual world of the Internet. The thesis identifies crucial factors that determine what constitutes a strong electronic identity solution and through these factors evaluates and compares the example solutions surveyed in the thesis. As the Internet become more pervasive in our lives; mobile devices are becoming the primary devices for communication and accessing Internet services. This has thus, raised the question of what sort of strong electronic identity solutions could be implemented and how such solutions could adapt to the future. To help to understand the possible alternate futures, a scenario planning and analysis method was used to develop a series of scenarios from underlying key economic, political, technological and social trends and uncertainties. The resulting three future scenarios indicate how the future of strong electronic identity will shape up with the aim of helping stakeholders contemplate the future and develop policies and strategies to better position themselves for the future

Análise comparativa na coaptação de tecido periodontal entre adesivo a base de cianoacrilato (super bonder) e fio de sutura.

Análise comparativa na coaptação de tecido periodontal entre adesivo a base de cianoacrilato (super bonder) e fio de sutura

Adesivos à base de cianoacrilato para síntese de tecido mole

FUNDAMENTOS - Adesivos teciduais têm sido muito usados para síntese de ferida, em função de ser um método indolor, rápido e de fácil execução. OBJETIVOS -Analisar e comparar compatibilidade dos adesivos, etil- cianoacrilato (Super Bonder) e butilcianoacrilato (Histoacryl), e a reparação de incisões em dorso de ratos entre o fio de sutura e os respectivos adesivos. MÉTODOS - Foram usados 15 ratos. Realizaram-se duas lojas cirúrgicas no dorso. em cada uma, foi implantado um tubo de polietileno (10mm x 1mm), os quais foram preenchidos com os adesivos Super Bonder (lado direito) e Histoacryl (lado esquerdo). As incisões, do lado esquerdo, foram coaptadas com Super Bonder, e as do lado direito, com Histoacryl. Uma incisão mediana, entre as duas incisões,foi realizada e suturada com fio de seda. Os animais foram mortos, depois de 7(sete), 35(trinta) e 120 (cento e vinte dia) dias. RESULTADOS: Os adesivos usados, no presente estudo, não promoveram reação inflamatória, quando usados para síntese das incisões. Porém, estes adesivos, quando implantados no subcutâneo, promoveram reação inflamatória até 120 (cento e vinte dia) dias, no entanto, a reação é mais intensa com Histoacryl. CONCLUSÕES: Super Bonder e Histoacryl permitem o processo cicatricial dos tecidos incisados; facilitam a sutura das incisões. Desta forma, estes podem ser utilizados para sínteses de feridas, lacerações ou incisões cutâneas.BACKGROUND: Tissue adhesives have been used for the synthesis of wound due to their painless application and quick and easy handling. OBJECTIVES: The purpose of this study was to analyze and compare the compatibility of the adhesives ethylcyanoacrylate (Super Bonder) and butyl-cyanoacrylate (Histoacryl), and to compare the reparation of incisions in the dorsum of rats with suture and the respective adhesives. METHODS: Fifteen rats were used. Two surgical pockets were created in their dorsum. A polyethylene tube (10mm x 1mm) was implanted in each one. Each tube was filled with the adhesives Super Bonder(left side) and Histoacryl (right side). The incisions on the left side were closed with Super Bonder, and the incisions on the right side, with Histoacryl. A median incision between the two other incisions was made and closed with braided silk suture. The animals were killed after, 7, 35 and 120 days. RESULTS: The adhesives used in the present study did not promote inflammatory reaction when used for the synthesis of incisions. However, when implanted subcutaneously, they caused an inflammatory reaction within 120 days. Reaction is more severe with Histoacryl. CONCLUSIONS: Super Bonder and Histoacryl can be used effectively in the healing of incised tissues; they aid in the suture of incisions. However, these adhesives can be used for the synthesis of wounds, lacerations or cutaneous incisions

Análise dos efeitos do adesivo dentinário ALL BOND 2 em dentina e polpa de ratos empregando análise histológica.

Análise dos efeitos do adesivo dentinário ALL BOND 2 em dentina e polpa de ratos empregando análise histológica

Effects of fibrin sealer and resorbable gelatin on the repair of osseous defects in rat tibia

Gelfoam® - a biologically resorbable gelatin sponge - has the function of restricting hemorrhage, providing platelet rupture, and supporting fibrin threads. Beriplast® - a fibrinogen-thrombin compound - is used to adhere tissues, to consolidate sutures and in hemostasis. The objective of this study was to perform a histological analysis of the effects of haemostatic agents on osseous repair. These materials were inserted into surgical sites in young rat right and left tibiae. After the observation periods of 7, 14, 30 and 45 days, according to the bioethic protocol, the animals were killed, the tibiae were removed and fixed in 10% formalin and decalcified in equal parts of formic acid and sodium citrate solutions. After routine processing, the specimens were embedded in paraffin for microtomy. Analysis of the results demonstrated that the haemostatic agents are effective in controlling hemorrhage; they stimulate osteogenesis, featuring a pattern of osseous tissue formation similar to the control pattern, although the amount of osseous trabeculae was superior, especially in the Gelfoam group in the periods of 7 and 14 days; 30 days after surgery, the delay in tissue healing in the control group in relation to the experimental groups started to decrease, and the control and experimental groups exhibited similar tissue repair after 45 days, when all the groups exhibited secondary osseous tissue